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Literature summary extracted from

  • Fukui, I.; Toyohara, H.; Ito, K.; Hamakubo, T.; Murachi, T.
    Molecular and catalytic characterization of intact heterodimeric and derived monomeric calpains isolated under different conditions from pig polymorphonuclear leukocytes (1988), Biochemistry, 27, 3260-3267.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.52 Ca2+ dimeric calpain I requires 0.002 mM for half-maximal activation and 0.01 mM for maximal activation. The 70000 Da monomeric calpain I requires only 0.001 mM Ca2+ for half-maximal activity Sus scrofa
3.4.22.53 Ca2+ half-maximal activation at 0.2 mM, maximal activation at 1 mM Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.52 29000
-
 1 * 83000 + 1 * 29000, an isolated 70000 Da calpain I, or monomeric artifact is also detected, SDS-PAGE Sus scrofa
3.4.22.52 83000
-
 1 * 83000 + 1 * 29000, an isolated 70000 Da calpain I, or monomeric artifact is also detected, SDS-PAGE Sus scrofa
3.4.22.53 29000
-
 1 * 80000 + 1 * 29000, SDS-PAGE Sus scrofa
3.4.22.53 80000
-
 1 * 80000 + 1 * 29000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.52 Sus scrofa
-
-
-
3.4.22.53 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.52 polymorphonuclear leukocyte
-
 Sus scrofa
-
3.4.22.53 polymorphonuclear leukocyte
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.52 casein + H2O
-
 Sus scrofa ?
-
 ?
3.4.22.53 casein + H2O
-
 Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.52 dimer 1 * 83000 + 1 * 29000, an isolated 70000 Da calpain I, or monomeric artifact is also detected, SDS-PAGE Sus scrofa
3.4.22.53 dimer 1 * 80000 + 1 * 29000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.52 additional information
-
 the 70000 Da monomeric calpain I is much less heat stable than the parent heterodimeric calpain I Sus scrofa
3.4.22.53 54
-
 pH 7.5, 10 min, 50% loss of activity Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.52 7.3
-
 monomeric calpain I Sus scrofa
3.4.22.52 7.5
-
 dimeric calpain I Sus scrofa
3.4.22.53 7.7
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.52 6.5 8 pH 6.5: about 35% of maximal activity, pH 8.0: about 50% of maximal activity Sus scrofa
3.4.22.53 6.5 8.5 pH 6.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.52 additional information
-
 the 70000 Da monomeric calpain I is less pH stable than the parent heterodimeric calpain I Sus scrofa